5 Seconds of Summer performed their song Good Girls on the show on October 2, 2014. They also appeared in a hashtag sketch entitled "#FallSongs" and talked about the first albums they each purchased while backstage.
5 Seconds of Summer performed their song Hey Everybody on the show on October 27, 2015 to celebrate the release of their second studio album, Sounds Good Feels Good. The episode only included their performance, but also featured Drew Barrymore and Stephen Moyer.
5 Seconds of Summer performed their song Want You Back on the show on April 11, 2018 to celebrate the release of their first single from their third studio album, Youngblood.